In vitro Callogenesis and Regeneration of Tomato (Lycopersicon esculentum Mill.)

Ghausia Aziz1, Hina Ghaffar1, Syeda Anam Akram1, Imran Shehzad1, Hafiz Muhammad Abdullah1, Amna Mughal1, Muhammad Nisar Anjum1 and Maria Rehman2*

1Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture Faisalabad. 2Department of Biotechnology, University of Sargodha

Abstract

Tomato (Solanum lycopersicum L.), is ranked at second in vegetables after potato. Although its demand and production is growing progressively, yet; biotic & abiotic stresses like high salt concentration, water shortage, high temperature, infectious diseases and nutrient deficiency are the main causes of its yield reduction. Many tissue culturing protocols and techniques have been practiced to develop such protocols which can enhance its rate of revival and prolong time of regeneration. Two cultivars of tomato Naqeeb and Nadir were selected to get explants and MS medium containing plant growth regulators including. Zeatin and IAA showed enhancement in regeneration frquency. Statistics of 192 explants was calculated after 4 weeks of culturing. Research was carried out on the basis of Trifactorial setup to study the result of genotype, different explant resources and media prepared and their collaborations individually & in combination, on in vitro competency of regeneration (count of shoots per ex-plant). Outcomes were calculated by means of Analysis of Varience, by comparing both varieties. Overall superiority of Tomato variety Naqeeb was observed. Fischer’s LSD test (Least Significant Difference) was used to calculate dissimlarities amongst the central values that are assumed arithmetically significant.