Abstract

Solanum melongena L. is an important vegetable in sub-tropical and tropical regions of the globe. It is prone to various biotic and abiotic stresses including diseases and insect pests being the most important ones. Many genes can be transferred into a plant at once because of the rapid improvement of genetic engineering tools. To apply the biotechnological approaches, first, we need to establish an efficient regeneration protocol. For tissue culture, different types of explants were tested for regeneration protocol optimization. For callus induction auxin and cytokines were used in a certain proportion. For regeneration, calli were shifted to regeneration media containing appropriate cytokines. The regenerated plantlets were shifted to rooting media containing IBA. Maximum callus fresh weight and callus induction efficiency were both achieved with 1 mg/L of 2, 4 D in combination with kinetin, BAP, and TDZ, respectively. Therefore, this research demonstrated progress in the development of regeneration and in vitro micro propagation of eggplant.

Keywords: Plant Tissue Culture, Callus, 2,4-D, IBA, Eggplant.